T-box 1 (or Tbx1) is a member of a gene family characterized by the presence of a region known as the T-box, which is a DNA-binding domain homologous to a region found in the protein product of the T gene.
The T, or Brachyury, gene was first identified as being responsible for a mutated mouse phenotype with a short, blunt-ended tail.
The T-box DNA-binding domain is 173-185 amino acids in length, and demonstrates strong evolutionary conservation across vertebrate species.
Seven mouse T-box genes have been identified: Tbx1-Tbx6 and Tbr-1.
Tbx1 gene expression has been studied by RTPCR and in situ hybridization (ISH) throughout murine embryonic development; high expression levels correspond with cranial neural crest cell migration (embryonic days 8.5 – 10.5) and therefore suggest an important role in the development of facial and glandular structures of the head and neck region, including the parathyroid and thymus glands.
The human ortholog of the mouse Tbx1 gene has been mapped to chromosome region.
a region commonly deleted in DiGeorge syndrome (DGS).
In humans, DGS is characterized by conotruncal cardiac defects, aplasia or hyperplasia of the thymus and parathyroid glands, palate abnormalities, developmental delay, and craniofacial dysmorphia.
Mouse models of heterozygous null Tbx1 mutations produce cardiovascular defects, disrupted neural crest and cranial nerve migration, middle and inner ear defects, and developmental abnormalities, modeling the human DiGeorge syndrome.
Applications:Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 1:20Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes mouse T-box 1 protein. On Western blots, a ~50kD band is identified in mouse testis, lung, and skeletal muscle homogenates. In mouse testis, an additional band at ~40kD, which may represent an isoform of Tbox 1, is also identified. Reactivity has been confirmed with mouse testis, lung, and skeletal muscle tissue homogenates.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to the internal region of the mouse T-box 1 (Tbx1) protein. Species sequence homology: three isoforms (A, B, C) of human T-box 1: 98%.
