84-1075-55　Syndecan 1 100ug　S9111-40A

特徴

• Syndecans 1-4 are heparan sulfate proteoglycans (HSPGs), which are cell-surface-associated proteins.
• Syndecans contain a small core protein with distinct functional domains to which are linked multiple glycosaminoglycan (GAG) chains, predominantly of the heparan sulfate variety.
• Syndecan synthesis is highly regulated and is dependent on both cell type and developmental state.
• Syndecans are involved in cell adhesion, tissue morphogenesis, and differentiation and regulation of cell responsiveness to soluble growth-regulatory compounds.
• These functions are attributed primarily to the glycan moieties.
• Syndecans also interact with growth factors such as fibroblast growth factor and epidermal growth factor.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:ELISA (Immunogen): 0.1-1ug/ml Western Blot (Human): 1-3ug/ml; Confirmed with human T47D, SW-480 and PC-3 cell lysates.
• A single band at ~90kD representing the glycosylated form of Syndecan 1 is observed and occasionally a band of ~31kD representing the unglycosylated form of Syndecan 1 may be observed.
• Immunohistochemistry (FFPE): 10ug/ml; For best results heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining.
• Confirmed with human gastric cancer and breast cancer tissues, as well as with mouse and rat stomach, small bowel and skin tissues.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.