SOCS-1, also called Janus kinase (JAK) binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is an SH2-domaincontaining protein.
It was shown to interact with all four Jak kinases (Jak1, Jak2, Jak3 and Tyk2) and is induced by and inhibits the signaling action of IL-3, IL-4, IL-6, growth hormone, LIF, prolactin, interferon gamma (IFN-g), and erythropoietin.
It plays an important role in the negative regulation of IFN-g signaling and in the differentiation of T-cells.
SOCS-1 mRNA is highly expressed in the thymus, to a much lower level in spleen, and not expressed in most other tissues.
Biochemical characterization as well as gene disruption studies indicate that SOCS-1 is an important negative regulator of the JAK-STAT signal pathway.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Controls:Mouse thymus cell lysates and HepG2 human hepatocellular carcinoma lysate.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity：Recognizes the human SOCS-1 protein at ~30kD. Species Crossreactivity: mouse Species Sequence Homology: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide derived from the C-terminal region of human, mouse or rat SOCS-1 protein
