PCDGF, also known as PC cell-derived growth factor, GP88, acrogranin, granulin precursor, epithelin precursor, or progranulin, is a glycoprotein and growth factor involved in tumorigenesis, the regulation of mitosis, non-neoplastic proliferative disorders, early embryonic development, cell division, cell invasion, cell survival, and hypothalamic differentiation.
Because PCDGF regulates multiple steps during carcinoma progression, it may represent a future therapeutic target.
PCDGF mediates resistance to the cancer drug tamoxifen, and promotes tumor growth of human breast cells.
Breast cancer samples that are estrogen receptor-positive and also overexpress PCDGF may therefore represent adverse consequences for patients if they are treated with tamoxifen.
PCDGF may promote metastasis and angiogenesis in human breast cancer cells, in addition to stimulating their proliferation and survival.
PCDGF staining is observed in breast carcinoma, whereas it is almost always negative in benign breast epithelium.
PCDGF expression is more common in invasive ductal carcinoma than in invasive lobular carcinoma.
PCDGF expression in human breast carcinoma correlates with clinicopathological variables such as tumor grade, proliferation index, and p53 expression, suggesting an important role of PCDGF in breast cancer pathogenesis.
PCDGF is also expressed in human multiple myeloma (MM) cell lines, human prostatic intraepithelial neoplasma (PIN) and invasive prostatic adenocarcinoma.
Applications:Suitable for use in Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Positive Control:Human MDA-MB-231 breast adenocarcinoma, PC-3 prostate adenocarcinoma (bone metastatic site), HeLa cervical carcinoma, and HT1080 fibrosarcoma lysates.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human PCDGF protein at ~64-88kD.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the C-terminal region of human PCDGF (PC cell-derived growth factor, GP88, acrogranin,granulin precursor, epithelin precursor, or progranulin).
