PKCs are products of multiple genes and alternative splicing that can be subdivided into several major classes, including conventional (c) PKC isoforms (alpha, beta I, beta II and gamma) and novel (n) PKC isoforms (delta, epsilon, eta and theta) and the atypical PKC (aPKC) subfamily members (sigma, iota and lambda).
Vertebrates express two atypical PKCs, sigma and lambda.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher
Positive Controls:Mouse spinal cord, adult and fetal mouse brain, and rat spinal cord homogenates.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes the ~74kD PKC lambda protein. Bands of unknown origin may be observed at ~85 and ~55kD in some homogenates.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the C-terminal region of mouse, rat and human PKC lambda.
