PDEF (prostate-derived Ets factor) is a 37.5-kD protein that acts as an androgen-independent transcriptional activator for the PSA (prostate specific antigen) promoter.
It also interacts directly with the DNA binding domain of androgen receptor and enhances androgen-mediated activation of the PSA promoter.
PDEF was shown to have transcript levels 192-fold higher in the peripheral blood of some breast cancer patients in comparison with normal individuals.
PDEF mRNA is alsofrequently over-expressed in human breast tumors as well as in human ovarian tumors.
In ovarian tumors, PDEF expression seems to down-modulate malignant potential, and thus provides a rationale to screen for drugs that induce PDEF expression in epithelial ovarian tumors.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Specific for human PDEF. On Western blots, it identifies a single band at ~37.5kD using LNCAP lysates. There is also a weak band at ~55kD which may be due to the glycosylated form of PDEF. Reactivity has been confirmed with human LNCAP prostate carcinoma cell lysates.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the internal region of human PDEF (prostate-derived Ets factor).
