The N-methyl-D-asparate (NMDA) receptor NR2B subunit has been implicated in modulating functions such as learning, memory processing, pain perception and feeding behaviors.
(1) It has also been suggested that NMDA receptor antagonists that selectively target the NR2B subunit may have anti-parkinsonian actions.
(2) The NR2B subunit has also been implicated inHuntington disease.
(3) Phosphorylation of Tyr1472 is largely dependent on the kinase Fyn, with phosphorylation events facilitated by the presence of PSD-95.
(4) Decreases in Tyr1472 phosphorylation of the NMDA receptor NR2B subunit have also been associated with the neuroprotective effects of lithium, which is a mood stabilizer.
(5)
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Specificity：Reacts with rat NMDA NR2B when it is phosphorylated at Tyr1472. On Western blots, it identifies a band at ~180 kD. In Western blots on rat brain two additional bands may be seen at ~115 and ~65 kD. Reactivity has been confirmed with rat brain homogenates. The labeling of the NR2B protein is blocked by the phosphopeptide but not by the dephosphopeptide. Based on 100% amino acid identity with mouse and human, crossreactivity is expected with mouse and human phospho-NMDA NR2B Tyr1472 proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Tyr1472 of rat NMDA receptor, NR2B subunit.
