The NFkB family of induced-activated transcription factors are dimers comprised of members of the NFkB /rel protein family.
Activation of NFkB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA, LPS, UV light, and others.
In most cell types, NFkB is present in the cytoplasm as a 50 kD (p50) subunit and a 65 kD (p65, rel A) subunit.
Inunstimulated cells, nuclear localization signals present on p65 are masked by members of the IkB family of inhibitory proteins.
Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosomedependant pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor.
In the nucleus, NFkB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA (Immunogen): 0.1-1ug/mlWestern Blot (Human and monkey): 1-3ug/mlImmunohistochemistry (FFPE) (human): 0.5-2ug/ml.
Heat-induced epitope retrieval (Citrate buffer, pH 6.0) is requiredImmunoprecipitation: 2-5ug/IPOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes the DNA binding subunit of human NFkB (also referred to as Rel A) at ~65kD.. Reactivity with other endogenous proteins has not been detected. Species Crossreactivity: monkey. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mk
Immunogen：A recombinant protein derived from the carboxyl terminal portion of the human NFkB protein.
