The capacity of the adult mammalian central nervous system (CNS) to repair lesions by axonal regeneration is very limited.
However, CNS axons can extend for long distances in peripheral nerve grafts.
CNS white matter selectively inhibits axonal outgrowth.
Several components of CNS white matter, NI35, NI250 (Nogo) and MAG, that inhibit axon extension have been described.
Neurite outgrowth inhibitor Nogo, also known as ASY and RTN-X, is a member of the reticulon (Rtn) family.
Research on Nogo may be important in finding ways to promote nerve regeneration after stroke or spinal cord injury.
Nogo induces apoptosis in various cancer cells when overexpressed so it may act to suppress tumor development.
The nogo gene encodes at least three major protein products (Nogo-A, -B and –C).
Nogo-A is the full-length protein (1192 aa).
Nogo-B is the intermediate isoform that lacks amino acids 186-1004 within the extracellular domain (373 aa).
Nogo-C, the shortest splice variant, also lacks amino acids 186-1004 but contains a smaller alternative N-terminal domain than Nogo-B.
Nogo-A is also known as Reticulon 4-A, is expressed by oligodendrocytes but not by Schwann cells and associates withthe endoplasmic reticulum.
Another names for rat Nogo-A include Foocen and Glut4 vesicle 20kD protein.
Nogo-A is a potent inhibitor of neurite growth and contributes to the failure of axonal regeneration in the adult CNS9.
The cloning of the human Nogo-A sequence shows that it may be a membrane-associated protein that consists of a putative large extracellular domain of 1024 amino acids with seven predicted N-linked glycosylation sites, two or three transmembrane domains and a short carboxy-terminal region.
The Nogo-A protein contains at least two active domains, NiG and Nogo-66 that mediate heir effects via an antagonistic regulation of the small GTPases RhoA and Rac1, resulting in activation of RhoA and suppression of Rac115.
The calculated molecular weight of rat Nogo-A is ~ 126kD, while the apparent molecular weight of the fully glycosylated form is ~ 180-190kD.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunoprecipitation: 7ug/ReactionOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Specific for rat Nogo-A. On Western blots for rat brain homogenates, it identifies a band at ~190kD. The faint bands at lower molecular weights may represent the deglycosylated form(s) or degradation product(s) of rat Nogo-A. Reactivity has been confirmed with rat brain homogenates. Based on amino acid sequence homolog, cross-reactivity withhuman is expected.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide derived from the internal region of rat Nogo-A (Reticulon 4-A, Foocen, Glut4 Vesicle 20kD Protein), which differs from the human sequence by only one amino acid.
