pGEX vectors are frequently utilized in bacterial systems to express polypeptides or entire proteins as fusions with glutathione S-transferase.
Using this fusion system, a polypeptide sequence is linked to the C-terminus of the 26 kD GST protein from Schistosoma japonicum.
The bacterially expressed GST-fusion proteins are purified by absorption with glutathione-agarose beads.
The fusion protein is subsequently eluted from the glutathione agarose with an excess of free glutathione.
Antibodies to GST are used to detect pGEX-expressed fusion proteins and as immuno-affinity reagents for purification of recombinant proteins.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (solid phase = antigen): 0.1-1.0ug/mlELISA (solid phase = antibody): 1.0ug/mlWestern Blotting: 1ug/mlOptimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, containing 0.05% sodium azide.
Specificity：Specific for the ~26kD GST protein from S. japonicum and is useful for detecting GST and GST-fusion proteins transcribed from pGEX and similar vectors. Cross-reactivity with endogenous proteins from E. coli bacteria has not been observed.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Purified glutathione S-transferase (GST) protein from Schistosoma japonicum expressed from a pGEX vector.
