In the mammalian central nervous system, glutamate and its receptors (GluR) are the major excitatory neurotransmitter and receptor, respectively.
Imbalances in glutamatergic function have been implicated in neuronal death following ischemia, hypoglycemia or anoxia, epilepsy, and in neurodegenerative disorders.
Based on their activation by different pharmacologic agonists GluR have been classified into two major superfamilies: the ligand-gated ion channel receptors referred to as ionotropic GluR (iGluR), and the G-protein-coupled receptors referred to as metabotropic GluR (mGluR).
The iGluR respond to selective agonists and are thus defined as the N-methyl-D-aspartate (NMDA) receptors, the alphaamino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA) responsive receptors (GluR1, 2, 3 and 4) and the kainate responsive receptors (GluR5 and 6).
GluR1 is phosphorylated on multiple sites, all are located on the C-terminal of the protein.
Ligand induced phosphorylation of GluR1 seems to be site-specific.
In one study, using transfected HEK cells and neurons in culture, cyclic AMP-dependent protein kinase specifically phosphorylates Ser845 of GluR11.
In addition, protein kinase C specifically phosphorylates Ser831 of GluR12.
The phosphorylation pattern of GluR1 by PKA and PKC is consistent with the study of AMPA-Rs in cortical neurins and GluR1 expressed in 293 cells.
These results suggested that the function of GluR1 can be regulated by protein phosphorylation.
Applications:Suitable for use in ELISA, Western Blot and Dot Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Dot Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control: Rat brain hippocampal homogenate.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes the ~100kD rat GluR1 protein, phosphorylated at Ser845. Does not react with the non-phosphorylated form of the GluR protein.
Isotype：IgG
Calc Applications Abbrev：DB E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic phospho-peptide derived from the rat GluR1 protein.
