Myotonic dystrophy (DM) is a highly variable multisystemic disease that leads to muscle wasting, myotonia, cataracts, heart block and neurobehavioral abnormalities.
Myotonic dystrophy is caused by a polymorphic expansion of trinucleotide (CTG) repeats in the 3’ untranslated region (UTR) of a c-AMP-dependent protein kinase (myotonic dystrophy protein kinase, DMPK) gene.
Strong nucleosome positioning signals created by this expanded repeat cause a reduction in gene expression within the region.
The molecular weight of DMPK is 80kD and it is expressed in the cytosolic fraction of skeletal muscles.
DMPK is localized at the gap junction level in the heart, specifically in the intercalated disc.
Heart transcripts of the DMPK (DMRB15) gene are subject to alternative splicing in both human and mouse
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity：Specific for the ~80kD DMPK Protein. Reactivity is confirmed with mouse heart and skeletal muscle homogenates.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide from the C-terminal region of the mouse Myotonic Dystrophy Protein kinase (DMPK) protein
