DARPP-32, a dopamine (DA) and cAMP-regulated ~32kD phosphoprotein that is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia,1 inhibits protein phosphatase I when it is phosphory on Thr34.
In contrast, when DARPP-32 is phosphorylated on Thr75, the protein acts as an inhibitor of PKA.
Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission2.
In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Specificity：Reacts with human, mouse, and rat DARPP-32 proteins phosphorylated at Thr34. On Western blots, it identifies the target band at ~32 kD. Reactivity has been confirmed with rat caudate slices incubated in the presence of forskolin.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from amino acids surrounding the phosphorylated Thr34 residue of DARPP-32.
