84-1075-11　Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3) 100ug　C7858-07C

特徴

• Connexin 46 (Cx46), also known as Gap Junction Protein Alpha-3 (GJA3), CAE3, CX46 and C2P3, maps to human chromosome 13q11-q12 and encodes a 46kD protein.
• Cx46, along with Cx50, is principally expressed in the lens of the eye.
• Cx46 mediates intercellular interactions during development and is necessary for the survival of neural crest cells.
• Cx46 forms gap junctions that connect lens fiber cells and are crucial for maintaining lens transparency and normal lens function.
• Mutations of Cx46 result in severe cataracts of the lens.
• Applications:Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1-3ug/ml.
• Identifies a single band at ~46kDImmunofluorescence: 3ug/mlImmunohistochemistry (Frozen): 3ug/mlNote: For IHC, mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, pH 7.6, 0.2% picric acid, and 1-, 2-, or 4% paraformaldehyde), and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4.
• Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.