84-1075-03　Claudin 10 (CLDN10) 100ug　C5838-60

特徴

• Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence.
• The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces.
• The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane.
• Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs.
• Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton mediated by ZO-1.
• Claudin 10 is concentrated at the tight junctions of epithelial cells of AQP1-positive tubules in the kidney cortex (proximal tubules).
• Claudin 10 is also detected at the tight junctions of epithelial cells of THP-positive tubules in the kidney medulla (thick ascending limb of Henle).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunohistochemistry: Frozen sectionsImmunohistochemistry (Paraffin): 1:10-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.