Claudin-8 is a tight junction protein expressed in several mouse distal nephron segments, suggesting that it may contribute to the paracellular barrier that protects transtubular Na+, K+, and H+ gradients in the distal nephron.
Caludin-8 decreases paracellular permeability to cations when overexpressed in MDCK-II cells, suggesting that it may function as a paracellular cation barrier.
Caludin-8 is also found in the thin ascending limb of Henle, a segment of the nephron that is known to be leaky to Na+.
Claudin-8 is expressed primarily in tight junctions along the entire aldosterone-sensitive distal nephron (ASDN) and in the late thin descending limb of Henle, consistent with its role as a paracellular cation barrier protein.
Claudin-8 is found in umbrella cell tight junctions in the bladder epithelium.
Expression of Claudin-8 has also been reported in the inner ear.
Applications:Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence/Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes mouse Claudin-8. Species Crossreactivity: rat and canine.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Ca Mo Rt
Immunogen：Synthetic peptide derived from the C-terminal region of mouse and rat Claudin-8.
