Claudin-4 belongs to the 20-member family of claudins, transmembrane proteins localized at epithelial and endothelial tight junctions.
Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux.
The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl– permeability.
In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels.
Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:ELISA: 0.1-1ug/mlImmunoprecipitation: 1-3ug/mlWestern Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes mouse Claudin 4. On Western blots a 22kD band is observed and a 44kD band representing dimerized protein may be observed. Reactivity has been confirmed with mouse and rat kidney homogenates. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IF IP WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminal region of the mouse Claudin-4 protein.
