The c-Fos oncoprotein is a member of the AP-1 family of transcription factors, which includes other Fos-related (FosB, Fra-1, Fra-2) and Jun-related (c-Jun, Jun B, Jun D) proteins.
The downstream effects of c-Fos induction are numerous and in addition to roles in proliferation, transformation and apoptosis, c-Fos has been implicated in the differentiation of several celltypes.
c-Fos also plays a role in the regulation of gene expression during proliferation and in the responses of postmitotic neurons to trans-synaptic stimulation.
c-Fos is also required for the development of a malignant tumor.
c-Fos is localized in the nucleus of normal human tissues.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Reacts with the ~55kD c-Fos protein. Additional and unidentified bands at ~65 and ~75kD were also observed in Western blots of EGF-stimulated A431 and T47D cell lysates. Reactivity has been confirmed in Western blotting with human U2-OS, SW480, T47D, EGF-stimulated A431, NCI-H69, and jurkat cell lysates. The 55kD target band was also identified in mouse NIH-3T3 and rat PC-12 cell lysates. Based on amino acid sequence homology, reactivity is also expected with porcine, bovine, cat, Chinese hamster, and golden hamster c-Fos proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Fe Po
Immunogen：Synthetic peptide derived from the C-terminal region of human c-Fos
