Aurora C1 is a member of the aurora/Ipl1p family of protein kinases that are important regulators of mitosis.2 Aurora C is a novel chromosomal passenger protein that, in coordination with Aurora B, regulates mitotic chromosome dynamics in human cells.3,4 Aurora C may play a role in organizing microtubules relating to the function of the centrosome/spindle pole during mitosis, and is localized to the centrosome from anaphase to cytokinesis.5 The expression of Aurora C is maximum at M phase.5 Aurora C is activated by directly associating with inner centromere protein (INCENP).3 Aurora C is expressed in normal testis6, and elevated expression levels are seen in a subset of cancer cells such as HepG2 and HeLa cells.5
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human placenta homogenates, and human JEG3, HEPG2, HeLa, HEK293, NGP96, SKNEP-1 and U251 cell lysates.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human Aurora C protein at ~32kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from an internal region of the human Aurora C (Serine/threonine-protein kinase 13, Serine/threonine kinase AIE2, Aurora/IPL1/Eg2 protein 2, Aurora/IPL1-related kinase 3) protein.
