Senile plaques and neurofibrillary tangles in brain are hallmarks of Alzheimer’s disease.
Mutations in the beta-amyloid precursor protein (beta-APP) are contributory factors to Familial Alzheimer’s Disease.
The primary constituent of senile plaques are deposits of the 4 kD amyloid beta-peptide, derived from amyloid beta-precursor proteins by internal proteolysis.
The major brain beta-APP isoforms (APP695, 751, 770) are integral membrane proteins with a single large N-terminal extracellular domain, a single transmembrane domain, and a small cytoplasmic C-terminal tail.
In the early 1990s, it was discovered that beta-APP is a useful marker for axonal injury.
beta-APP is carried by fast anterograde transport vesicles to distal sites in the axon with the beta-APP C-terminus residing in the cytoplasm and the N-terminus in the vesicle.
Upon axonal injury, beta-APP will pool in areas of impaired transport.
beta-APP has been used as a marker of axonal injury in many studies of different pathologies, including: head trauma, HTLV-I-associated myelopathy, multiple sclerosis, shaken baby syndrome, and encephalomyelitis among others.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorscence and Immunoprecipitation.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human β-Amyloid Precursor Protein. Reacts with full-length and N-terminal truncated forms of β-Amyloid Precursor Protein. Detects the C-terminal membrane-anchored fragment of β-Amyloid Precursor Protein that remains after alpha- or beta-secretase cleavage. Does not detect the beta-APP product N-terminal to the gamma-secretase cleavage site. Species Crossreactivity: mouse, pig and rat
Isotype：IgG
Calc Applications Abbrev：E IF IP WB
Calc Crossreactivity：Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to 22aa located at the C terminus of human β-Amyloid Precursor Protein. Species Sequence Homology: monkey, guinea pig, chicken
