84-1074-79 ［取扱停止］Fas Ligand (FASL) (BSA Free) 50ug F0019-65H1
特徴
- Applications:Suitable for use in ELISA and Western Blot.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1:1000-1:5000(ECL).
- We recommend the use of 0.5-1% milk in diluents used for primary antibody and secondary antibody-enzyme conjugates to suppress background.
- Rat, mouse and human FASL have 278, 279, and 281aa residues, respectively (Calculated MW of ~32kD).
- Western blot of the oocyte has detected a specific 31-kD band (1).
- A 40kD FASL has been detected in mouse T cells (1).
- An alternate detecting reagent, mFAS-Fc (1) also detected a cell surface protein of about 40kD.
- ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
- Control Peptide:F0019-65R
- Storage and Stability:May be stored at 4°C for short-term only.
- For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C.
- Aliquots are stable for at least 12 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Further dilutions can be made in assay buffer.
仕様
- Size：50ug
- Host：rabbit
- Source Antibody：human
- Grade：Affinity Purified
- Purity：Purified by immunoaffinity chromatography
- Form：Supplied as a liquid in 100mM Tris, pH 7.5. No stabilizing proteins added. Also available with BSA. See F0019-65H.
- Specificity：Recognizes human FASL. Species sequence homology: mouse and rat-92%.
- Isotype：IgG
- Calc Applications Abbrev：E WB
- Calc Crossreactivity：Hu
- Immunogen：13-aa peptide from human FASL near the C terminus (KLH).
- Shelf Life：1year
- EU Commodity Code：30021010
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