84-1074-45　［取扱停止］Pax6 (Paired Box Gene 6, AN2, D11S812E, MGDA, WAGR) discontinued 50ug　P3113-84A

特徴

• Retinal progenitor cells (RPCs) retain the ability to generate different retinal cell types.
• RPCs appear to be retained in a progenitor state by the action of Notch-Delta signaling and downstream effectors of Notch, like the bHLH transcription factor Hes1.
• The most uncommitted RPCs (retinal stem cells) in the ciliary margin of the Xenopus retina were shown to coexpress the transcription factors Pax6, RX1, and Six3 prior to the activation of neurogenic genes (Notch, Delta, bHLH) and the subsequent steps toward retinal neurogenesis.
• Pax6 is required for the multipotent state of retinal progenitor C.
• Upon Pax 6 inactivation, the potential of RPCs becomes entirely restricted to only one of the cell fates normally available to RPCs resulting in the exclusive generation of amacrine interneurons.
• Pax6 has been found to directly control the transcriptional activation of retinogenic bHLH factors that bias subsets of RPCs towards different retinal cell fates, thereby mediating the full retinogenic potential of RPCs.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA (Serum Titer): 1:42,500Western Blot: Preliminary results: A549 cell: 75kD, Mouse fetus: 40, 50, 70kD, Human cerebellum: 65kDOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.