Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases.
The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3).
VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins.
VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha.
VAV 3 is also involved in activating JNK, p21-activated kinase (PAK) and PLC lambda, implicating a critical role in cytoskeletal rearrangement.
VAV 3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro.
Tyrosine 173 is the regulatory phosphorylation site of VAV 3.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml detected Vav3 in RIPA lysates from HeLa and Jurkat cells
Immunoprecipitation: Reported to immunoprecipitate Vav3 from Jurkat cell lysate and SU86.86 pancreatic tumor cell lysate
Immunocytochemistry: Reported to detect Vav3 in Jurkat and SU86.86 pancreatic tumor cells
Immunohistochemistry: Reported to detect Vav3 in pancreatic adenocarcinoma sections.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes human Vav3, Mr ~98kD. Additional non-specific bands detected in long exposures at Mr ~38-40kD. A non-specific band at Mr ~95kD was detected in Jurkat lysates.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa566-591 (NSGEQGTLKLPEKRTNGLRRTPKQVD) of human Vav3 (KLH).
