Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: A 1:500-1:1000 detected TTF-1 in nuclear extract from H441 human lung epithelial cells
Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate TTF-1
Immunohistochemistry: Has been reported by an independent laboratory to detect TTF-1 in 4% paraformaldehyde fixed human and mouse lung adenocarcinoma tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:rat
Grade:Serum
Purity:Serum
Form:Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity:Recognizes rat TTF-1, Mr 42kD. Additional non-specific proteins also detected in longer exposures. Species Crossreactivity: mouse and human.
Isotype:IgG
Calc Applications Abbrev:IHC IP WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:6X His fusion protein corresponding to full-length TTF-1 (Thyroid transcription factor 1, also known as Thyroid nuclear factor 1, Homeobox protein Nkx-2.1).