Sam68, the 68kD Src substrate associated during mitosis, is an RNA-binding protein with signaling properties that contains a GSG (GRP33, Sam68, GLD-1) domain.
Two Sam68-like-mammalian proteins, SLM-1 and SLM-2 have an ≈70% sequence identity with Sam68 in their GSG domain.
SLM-1 and SLM-2 have the characteristic Sam68 SH2 and SH3 domain binding sites.
SLM-1 is an RNA-binding protein that is tyrosine phosphorylated by Src during mitosis.
SLM-1 bound the SH2 and SH3 domains of p59fyn, Grb-2, phospholipase Cg-1 (PLCg-1), and/or p120rasGAP, suggesting it may function as a multifunctional adapter protein for Src during mitosis.
SLM-2 is an RNA-binding protein that is not tyrosine phosphorylated by Src or p59fyn.
Moreover, SLM-2 did not associate with the SH3 domains of p59fyn, Grb-2, PLCg-1, or p120rasGAP, suggesting that SLM-2 may not function as an adapter protein for these proteins.
The identification of SLM-1 and SLM-2 demonstrates the presence of a Sam68/SLM family whose members have the potential to link signaling pathways with RNA metabolism.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
