Arginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing.
PRMTs are divided into two types defined by their activity.
Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues.
Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues.
Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins.
PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer.
Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.
PRMT5 (aka JBP1 Janus Binding Protein1) is a member of a family of enzymes that deposit methyl groups on arginine residues of proteins, including both histone and non-histone substrates.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation: Reported to immunoprecipitate active PRMT5 from HeLa cell lysate
Western Blot: 0.5-1ug/ml in RIPA lysates from 3T3/A31, NIH 3T3, HeLa, WI-38 and Jurket cellsOptimal dilutions to be determined by the researcher.
Positive Control Included:J9000: Jurkat cell lysate.
Add 2.5ul of 2-mercaptoethanol/ 100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes human PRMT5, Mr ~70kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa60-79, KNRPGPQTRSDLLLSGRDWN, of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.
