The methylation of arginine is mediated by the Protein Arginine Methyl Transferase (PRMT) family of enzymes.
These enzymes transfer the methyl group from S-adenosyl-Lmethionine to the guanidino nitrogen atoms of an arginine residue.
Arginine methylation was found to be an important modification in signal transduction, transcription, RNA transport, and splicing.
PRMT1, also known as HRMT1L2 and IR1B4, was isolated through its interaction with BTG1 and TIS21, proteins that are important in cell quiescence.
PRMT1 is a 361 amino acid protein; its splicing variants differ at the N-terminus, giving rise to three isoforms of 343, 361, and 347 amino acids (variants 1, 2, and 3 respectively).
Proteins involved in the metabolism of RNA are substrates for PRMT1, among them Sam68.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml of this lot detected PRMT1 in RIPA lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:Jurkat lysate.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes the PRMT1 doublet, Mr ~42- 44kD. A non-specific protein was also detected, Mr ~120kD. Species Crossreactivity: Human and mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa298-318 (KTGEEIFGTIGMRPNAKNNRD) of human protein arginine methyltransferase 1 (accession number CAA71765). The immunizing sequence is identical in mouse.
