p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas.
Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene.
The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression.
p53's role in the multistep process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors
Expression correlates with poor prognosis in breast cancer.
In colonic cancer p53 expression seen in 47% of tumors and 9% of colonomas.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 detects GST-p53 acetylated by recombinant p300, HAT domain in an in vitro acetyl transferase assay.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Specific for human p53 acetylated in vitro by recombinant p300, and for peptide containing acetyllysine 373 but not for the peptide containing acetyllysine 320. Modest crossreactivity for p53 acetylated in vitro by recombinant PCAF.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa367-379 of human p53 (SHLKSK[AcK]GQSTSR, where AcK denotes acetyllysine). Species Sequence homology: Mouse and rat. The specificity was demonstrated with synthetic acetylated peptides.
