Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase.
Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes.
Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells
Immunoprecipitation: 10ul immunoprecipitates p47-phox from 500ug of HL-60 RIPA lysate
Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 5mM EDTA, 0.02% sodium azide.
Specificity：Recognizes human p47-phox, Mr 47kD. Reported crossreactivity with bovine.
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Bo Hu
Immunogen：GST fusion protein corresponding to full-length human p47-phox
