Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase.
It generates NO in blood vessels and is involved with regulating vascular function.
A constitutive Ca2+ dependent NOS provides a basal release of NO.
eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml detects eNOS/NOS III in RIPA lysates from HUVEC cells
Optimal dilutions to be determined by the researcher.
Recommended Control:Platelets, placenta, liver and kidney
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity：Recognizes human eNOS/NOS III, Mr ~135kD. Species Crossreactivity: bovine
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu
Immunogen：Synthetic peptide corresponding to aa1181-1194, C-QERQLRGAVPWAFD of human eNOS/NOS III, conjugated to KLH. Species Sequence Homology: mouse, guinea pig, porcine, rat and canine; 10/11aa.
