Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/ml detected a Myc epitope tagged protein in a transfected Cos-1 cell lysate.
1ug/ml of a previous lot detected endogenous Myc in HeLa nuclear extract.
A lysate of transfected Cos-1 cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myc Tag (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Myc epitope tagged protein (~66kD)Immunocytochemistry: Reported to detect Myc-tagged proteins in transfected cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN)
