Recommended Dilution:Western Blot: 0.5-2ug/ml detected MeCP2 in mouse heart nuclear extracts and a previous lot detected MeCP2 in mouse brain nuclear extracts and L6 nuclear extracts
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Specificity：Recognizes MeCP2, Mr 75kD. A protein at ~75kD was also observed in HeLa nuclear extract. This protein was competed by pre-incubation of the antibody with the immunogenic peptide, suggesting that some HeLa strains contain MeCP2 or a related protein, contrary to a previous report. Species Crossreactivity: Mouse and rat. Predicted to crossreact with human based on the conservation of the immunogenic sequence.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes
