84-1073-97　Lysine, Acetylated (Acetyl-Lysine) 100ul　L9058-03

特徴

• In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription.
• Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression.
• The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process.
• Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains.
• The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain.
• Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 dilution detected Acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells.
• Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones.
• GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF
• Immunoprecipitation: 5ul immunoprecipitated in vitro acetylated recombinant p300
• Optimal dilutions to be determined by the researcher.
• Positive Control:TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.