Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells.
Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes.
These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Applications:Suitable for use in Western Blot, Dot Blot, Immunoprecipitation and Chromatin IP (ChIP).
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 dilution of this lot detected Histone H4 (Lys8) on 10ug of sodium butyrate and untreated HeLa cell lysate
Chromatin Immunoprecipitation (ChIP): 0.1-0.5ul of a representative lot of antibody immunoprecipitated chromatin from yeast
Optimal dilutions to be determined by the researcher.
Positive Control: Sodium butyrate treated HeLa cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes acetyl-Histone H4 (Lys8), Mr 14kD. Does not detect Histone H4 from yeast containing a Histone H4 K8R substitution. Species Crossreactivity: Yeast and human.
Isotype：IgG
Calc Applications Abbrev：ChIP DB IP WB
Calc Crossreactivity：Hu Ye
Immunogen：Synthetic peptide corresponding to aa3-13, RGKGGAcKGLGKG-C of yeast Histone H4 acetylated on lysine 8 conjugated with ovalbumin, with a C-terminal cysteine added for conjugation purposes.
