Applications:Suitable for use in Western Blot and ChIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 detected acetyl-Histone H4 (Lys5) in acid extracts from sodium butyrate treated HeLa cells ChIP: An independent laboratory has shown that this antibody preferentially immunoprecipitates chromatin from wild type yeast, compared to yeast containing a histone H4 K5R substitution.(1)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a liquid in 0.01% thimerosal.
Specificity:Recognizes acetyl-Histone H4 (Lys5). Species Crossreactivity: Yeast and human. Broad species crossreactivity is expected.
Isotype:IgG
Calc Applications Abbrev:ChIP WB
Calc Crossreactivity:Hu Ye
Immunogen:KLH-conjugated linear peptide corresponding to the N-terminus of Histone H4 acetylated at Lys5.