Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells.
Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes.
These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Applications:Suitable for use in Dot Blot and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:3000 detects methylated Histone H3 in acid-extracted proteins from untreated HeLa cells.
Did not detect recombinant Xenopus laevis histone H3 purified from E.
coli
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Immunodepleted serum; Serum was passed over immunoaffinity column containing the non-modified peptide.
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes human methylated Histone H3 (Lys36), Mr 17kD. Does not crossreact with peptides containing dimethyl histone H4 Lys20, nor Histone H3 Lys4, Lys9, or Lys27. Species Crossreactivity: mouse, chicken, Drosophilia and yeast.
Isotype：IgG
Calc Applications Abbrev：DB WB
Calc Crossreactivity：Ch Dr Hu Mo Ye
Immunogen：Synthetic peptide corresponding to aa30-38, CPATGGV(dimethyl-K)KP, of Histone H3 with a N-terminal Cysteine added for conjugation (KLH). The immunizing sequence is conserved from Tetrahymena to human.
