Applications:Suitable for use in Western Blot, Beadlyte Histone-Peptide Specificity Assay and Chromatin IP.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:5000 detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells Beadlyte Histone-Peptide Specificity Assay: 1:1000-1:5000 were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres.
No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected
Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes acetyl-histone H3 (Lys14), Mr ~17kD. An additional unknown protein was detected at Mr ~33kD. Species Crossreactivity: Yeast and human. Broad species crossreactivity is expected.
Isotype：IgG
Calc Applications Abbrev：ChIP WB
Calc Crossreactivity：Hu
Immunogen：Ovalbumin-conjugated, synthetic peptide (KSTGGAcKAPRK-C) corresponding to aa9-18 of yeast histone H3 acetylated on lysine 14, with a C-terminal cysteine added for conjugation purposes.
