Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells.
Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes.
These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Applications:Suitable for use in Western Blot, ChIP and Beadlyte Histone-Peptide Specificity Assay.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000-1:10,000 detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells
Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9
Beadlyte Histone-Peptide Specificity Assay: 1:1000-1:5000 was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres.
No crossreactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed
Optimal dilutions to be determined by the researcher.
Positive Control:TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes acetyl-histone H3 (Lys9), Mr 17kD. An additional unknown protein was detected at Mr 23kD. Species Crossreactivity: human and Yeast. Broad species crossreactivity is expected.
Isotype：IgG
Calc Applications Abbrev：ChIP WB
Calc Crossreactivity：Hu Ye
Immunogen：Ovalbumin-conjugated, synthetic peptide (KQTARAcKSTGGK-C) corresponding to aa4-14 of yeast histone H3 acetylated on lysine 9, with a C-terminal cysteine added for conjugation purposes.
