Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Applications:Suitable for use in Dot Blot, Western Blot, Peptide Inhibition Assay.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:6000.
Detects methylated Histone H3 (Lys9) in acid- extracted proteins from HeLa cells.
Did not detect unmethylated recombinant Histone H3
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum depleted of non-methyl reactive antibodies
Form：Supplied as a liquid containing 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes Histone H3 containing trimethyl-lysine 9 and, to a lesser extent, dimethyl-lysine 9. 10uM of a Histone H3 peptide containing trimethyl-lysine 9 abolished detection of Histone H3 by anti-trimethyl Histone H3 (Lys9) in immunoblot analysis of HeLa acid extracts. Slight signal reduction was observed with 10uM of a Histone H3 peptide containing dimethyl-lysine 9.Slight crossreactivity with Histone H3 peptides containing dimethyl-lysine 9 and trimethyl-lysine 27 was detected using Beadlyte Histone Peptide Assay. Slight crossreactivity with Histone H3 peptide containing dimethyl-lysine 9 was detected by peptide Dot Blot analysis.
Isotype：IgG
Calc Applications Abbrev：DB WB
Calc Crossreactivity：Hu
Immunogen：BSA-conjugated, synthetic peptide containing the sequence …AR[me3K]S… in which me3K corresponds to trimethyl-lysine 9 of human Histone H3.
