Recommended Dilution:Western Blot: 1:40,000-1:80,000 detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells.
Unacetylated recombinant Histone H2B was not detected
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity：Recognizes acetylated Histone H2B, Mr ~15kD. Does not recognize acetylated Histone H2A or unacetylated recombinant Histone H2B protein. Species Crossreactivity: Human. Predicted to crossreact with mouse, rat and bovine based on sequence homology.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：KLH-conjugated, synthetic peptide (…S[AcK]SAPAPK[AcK]GS[AcK]KAIT[AcK]AQ…) in which AcK corresponds to acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B. The immunizing sequence has 24 out of 26 identical amino acids in mouse, rat and bovine.
