H2AX is involved in DNA repair and the maintenance of genomic stability.
although its precise role is still to be defined.
H2AX is phosphorylated soon after the appearance of double-stranded breaks.
and has been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways.
H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins.
Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.
Applications:Suitable for use in Immunohistochemistry, Western Blot and Peptide Inhibition analysis.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:2000-1:10,000 detected histone H2A.X in acid-extracted proteins from HeLa cells.
The antibody did not detect recombinant Histone H2A
Immunohistochemistry (paraffin): 1:1000 detected Histone H2A.X in human pancreas and human colon tissue sections
Peptide Inhibition Analysis: 1 and 10uM of the immunizing histone H2A.X peptide abolished detection of histone H2A.X by this lot in immunoblot analysis of HeLa acid extracts
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa acid extracts
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes human histone H2A.X, Mr 15kD. Targets an epitope with in 15 amino acids from the C-terminal region.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa121-135, CSATVGPKAPSGGKKA, from human histone H2A.X, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 13/15 identical amino acids in mouse.
