84-1073-78 GRAP2 (GRB2 Related Adaptor Protein 2, GRB2 Like Protein, GADS, GRB2 Related Protein With Insert Domain, GRB2L, GRBLG, GrbX, Grf40, GRID, Growth Factor Receptor Binding Protein, GRPL, SH3-SH2-SH3 Adaptor Molecule) 200ug G8959-11
84-1073-78 GRAP2 (GRB2 Related Adaptor Protein 2, GRB2 Like Protein, GADS, GRB2 Related Protein With Insert Domain, GRB2L, GRBLG, GrbX, Grf40, GRID, Growth Factor Receptor Binding Protein, GRPL, SH3-SH2-SH3 Adaptor Molecule) 200ug G8959-11
This gene encodes a member of the GRB2/Sem5/Drk family.
This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling.
Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains.
This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains.
Transcript variants utilizing alternative polyA sites exist.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/ml detects GRAP2 from a Jurkat RIPA cell lysate
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:J9000 Jurkat Cell Lysate.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Use 20ug per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:Recognizes GADS, Mr 40kD. Species Crossreactivity: human and mouse.
Isotype:IgG
Calc Applications Abbrev:IP WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide corresponding to aa174-188 of mouse GADS (GEEIRPSVNRKLSDH) (KLH). Species sequence homology: human (14/15)