Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms.
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas.
Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family.
A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx1,2.
Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway.
Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways (1,2).
Applications:Suitable for use in Immunocytochemistry, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: 5ul immunoprecipitates Daxx from 150ug of total soluble protein
Western Blot: 1:5000-1:1000 detects Daxx in HeLa nuclear extract.
mmunocytochemistry: 1:100 shows positive immunostaining for Daxx in human cells fixed with 3.7% formaldehyde
Optimal dilutions to be determined by the researcher.
Positive Antigen Control:HeLa nuclear extract.
Add an equal volume of Laemmli reducing sample buffer to 10ul of extract and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced extract per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity：Recognizes Daxx, Mr ~110kD. An unknown band was also detected, Mr ~150kD. The identity of the Mr ~110kD band as Daxx was confirmed by an independent laboratory using a siRNA strategy. Species Crossreactivity. Human, mouse, rat and Chinese hamster.
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hm Hu Mo Rt
Immunogen：GST fusion protein corresponding to residues 472-740 of human Daxx
