84-1073-74　Citrulline 100ul　C5806-02

特徴

• The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation.
• Citrulline promotes energy and assists with the immune system.
• This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine.
• It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 detected Citrulline on 10ug of human cornified cell lysate.
• The human cornified cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with C5086-02.
• Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system
• Immunohistochemistry: 1:300 detected Citrulline in human skin tissue
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-46E: IgG, H&L (HRP) Pab DkxRbI1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab DkxRb
• Positive Control: Human cornified cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.