Applications:Suitable for use in Western Blot, Immunoprecipitation and IP-kinase assay.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/ml detected cdk2 in nuclear extracts from HeLa cells
Immunoprecipitation: 2-4ug immunoprecipitated cdk2 from 500ug of HeLa nuclear extract
Immunoprecipitation Kinase Assay: 4ug ￥immunoprecipitated active cdk2 from HeLa nuclear extract as shown by the ability to phosphorylate Histone H1
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity：Recognizes human p33cdk2 protein kinase, Mr 33kD. Species Crossreactivity: Human. Predicted to crossreact with mouse, rat, non-human primates and hamster.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa287-298 (CQDVTKPVPHLRL) of human cdk2, with a cysteine added for conjugation purposes.
