84-1073-71　BRCA1 (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer Susceptibility Protein 1) 100ul　B2709-03

特徴

• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:5000-1:10,000 dilution of this lot detected BRCA1 in HeLa nuclear extract
• Immunohistochemistry: This antibody has been reported by an independent laboratory to detect BRCA1 in breast cancer tumor sections.
• Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate BRCA1
• Optimal dilutions to be determined by the researcher.
• Included Positive Antigen Control:HeLa nuclear extract.
• Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation.
• Load 20ug of reduced lysate per lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.