84-1073-70　AUF1 (A+U Rich RNA Binding Factor) 200ug　A4165-04

特徴

• AUF1 Is a bcl-2 A + U-rich element-binding protein involved in bcl-2 mRNA destabilization during apoptosis.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.01-1ug/ml Detects AUF1 in RIPA lysates from HeLa nuclear extract and A431 cells.
• HeLa nuclear extract cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A4165-04 (0.1ug/ml).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
• Optimal dilutions to be determined by the researcher.
• Positive Control Included:H1838: HeLa nuclear extract.
• Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation.
• Load 20ug of reduces lysate per lane for minigels.
• Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.