Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: A 1:500-1:1000 detects methylated p60 (unidentified protein) and Sm protein B, B’ and D in RIPA lysates from Jurkat cells.
Jurkat cell lysate was resolved by electrophoresis, transferred to PVDF and probed with A3550-07C (1:1000).
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Detects bands at 60, 30, 32 and 16kD, corresponding to p60, Sm B, B’ and D, respectively.
Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate proteins containing symmetric dimethyl-arginine.
Immunocytochemistry: 1:500.
Reported by an independent laboratory to immunostain Cajal bodies in the nuclei of HeLa, WI-38 and NIH/3T3 cells fixed with paraformaldehyde and permeabilized with triton
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:J9000: Jurkat lysate.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes proteins that contain multiple symmetrically dimethylated arginines, including the Sm protein B, B’ and D. Does not recognize nonmethylated peptides, peptides containing asymmetrically dimethylated arginines, or a peptide containing a single symmetric dimethyl-arginine. Species Crossreactivity: human and mouse.
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G in which (R*) denotes symmetric dimethylation of arginine). Conjugated to KLH.
