Arginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/ml of this lot detected numerous proteins including SmD3 in RIPA lysates from Jurkat cells.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:J9000: Jurkat lysate.
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes SmD3 at ~16kD, and other proteins that contain arginines that are symmetrically dimethylated. Specificity of symmetrically dimethylated arginine was determined by ELISA with unmodified and symmetrically dimethylarginine containing peptides. Species Crossreactivity: human, mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa99-119, KAAILLAQVAAR*GR*GR*GMGR*G (which R* denotes symmetric dimethylation of arginine) of small nuclear ribonucleoprotein SmD3, conjugated to KLH.
