RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus.
The RELM family consists of resistin (also called FIZZ3), RELM-alpha (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma.
Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents.
RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool.
Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment.
Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant.
The physiological function of RELM-beta is not known.
High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.
Applications:Suitable for use in ELISA, Western blotting.
Titer: Defined by indirect ELISA is >1:100,000 for antibody concentration1mg/ml, 25ng of antigen are coated per well, and is then defined at a point ofmaximal decrease of the titration curve.
Reconstitution: Add 0.1ml of deionized water and let the lyophilized pellet dissolve completely.
Slight turbidity may occur after reconstitution, which does not affect activity ofthe antibody.
In this case clarify the solution by centrifugation.
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Immunoaffinity chromatography on a column with immobilized recombinant human RELM-beta.
Form：Sterile filtered and lyophilized from 1mg/ml in 0.05M phosphate buffer, 0.1M NaCl, pH 7.2.
Specificity：C-terminal His-Tagged fusion protein produced in E. coli, it is a 11kD protein containing 90 amino acid residues of the human RELM beta and 12 additional amino acid residues – His-Tag (underlined). The amino acid sequence of the recombinant human RELM beta is 100% homologous to the amino acid sequence of the human RELM beta without signal sequence.
